We interest in understanding the spatiotemporal regulation of cytoskeleton. Cells can move spontaneously in response to extracellular signals. For example, neutrophils detect substances released from bacteria and move autonomously toward the bacteria to prey on them, thereby preventing infection . In this way, cells respond to extracellular signals and remodel the arrangement of protein filaments of the cytoskeleton at the right place and time. The resulting spatiotemporal cytoskeletal rearrangement alters cellular morphology and generates the driving force for cellular motility. We are interested in understanding the regulation of spatial and temporal cytoskeletal remodeling in cell migration. Additionally, the timing of cytoskeletal remodeling controls vascular permeability, which regulates the exchange of substances between blood vessels and tissues. Therefore, we aim to elucidate the mechanism of vascular permeability control that regulates the exchange of substances between blood vessels and tissues and is in turn regulated by the timing of cytoskeletal remodeling.